Whole Genome Sequencing for Bacteria and Fungi

 

Introduction

Bacterial and fungal whole genome re-sequencing is a critical tool to complete the genomes of known bacterium and fungi, as well as to compare multiple genomes or to map genomes of new organisms. It is of great importance to sequence entire genomes of bacterium and fungi to generate accurate reference genomes, to do microbial identification and other comparative genome studies. Compared with traditional methods like PCR, next generation sequencing (NGS) excels in the way of multiplexing vast number of samples, providing identifications of SNP/InDel/CNV/SV with great reliability, turnaround time and cost performance. Molsys Scientific offers both reference based and De novo genome assembly workflow.

 

 

 

 

 

 

 

 

 

 

Whole Genome Sequencing for Higher Eukaryotes

 

Introduction

Human whole genome sequencing (hWGS) enables researchers to catalog a genetic constitution of individuals and capture all variants (single-nucleotide variations (SNVs), insertions and deletions (InDels), copy number variations (CNVs), and large structural variants (SV) present in a single assay. Equipped with the powerful Illumina NovaSeq 6000 system, Molsys is capable of sequencing human
genomes as well. With the addition of Oxford Nanopore and PacBio Systems, Molsys also provides hWGS services with more complete and accurate characterization of human genome and complements missing sequencing reads, especially in highly polymorphic and highly repetitive regions from short reads sequencing.

 

Applications

  • Identification of pathogenic organisms

  • Genome projects from population genomics

  • Surveillance and investigation of outbreaks

Data analysis

  • Genome assembly and annotation

  • SNPs and InDels calling and gene prediction

  • Secondary analysis includes options for usage of different algorithms and comparative analysis

We offer this service on the below mentioned platforms. Kindly note the sample specifications.

Illumina Novaseq 6000

Sample Type - Amount (Qubit®)

  • Genomic DNA

      Amount (Qubit®) ≥ 200 ng, Purity - OD 260/280 = 1.8 - 2.0

  • Genomic DNA (PCR free)

       Amount (Qubit®) ≥ 1.5 μg

  • Genomic DNA from FFPE

       Amount (Qubit®) ≥ 0.8 μg

 

PacBio Sequel I/II

Sample Type - Amount (Qubit®)

  • HMW Genomic DNA

      Amount (Qubit®) ≥ 10 μg (for Sequel I)
      Amount (Qubit®) ≥ 30 μg (for Sequel II)

      Purity - OD 260/280 = 1.8 - 2.0;
      Purity - OD 260/230 = 2.0 - 2.2;
Fragments should be ≥ 30 Kb for Sequel I, ≥ 60 Kb for Sequel II

 

Nanopore PromethION

  • HMW Genomic DNA

      Amount (Qubit®) ≥ 10 μg

      Purity - OD 260/280 = 1.8 - 2.0;
      Purity - OD 260/230 = 2.0 - 2.2;
      Fragments should be ≥ 30 Kb

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