Amplicon Metagenomics

Amplicon or target sequencing uses specific primers for marker genes

e.g., 16S, 18S, ITS that targets a specific gene or region in the genome of any species.

Shotgun Metagenomics

Whole genome metagenomic sequencing is often called Shotgun sequencing, because it takes a “shotgun approach” and aims to look at all the accessible DNA in a microbiome sample for mixed community