
Introduction
Epigenetic regulation includes modulating the transcriptional accessibility of genes through nucleosome positioning and chromatin remodeling. ATAC-Seq is a rapid assay that allows analysis of epigenetic profiles across the genome by identification of regions that have open or accessible chromatin states. A good alternative to ChIP-seq when beginning epigenetic studies is Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq). ATAC-seq is a common assay being utilized to survey the landscape genome-wide of chromatin assembly, understand accessibility to regions, discover transcription factor binding sites, gene regulation, and more. ATAC-seq has an advantage over other epigenomic assays as it requires a small amount of starting input. Molsys Scientific now offers the entire ATAC-Seq workflow, from library preparation to data analysis. ATAC-Seq has a higher signal-to-noise ratio and lower input requirements than other similar approaches.

Applications
•Global view of gene activity
•Novel gene and splice variant discovery
•Chromosomal rearrangement analysis
•Whole mRNA transcript expression analysis
Data Analysis
Primary Analysis
•Global methylation profiles
•Specific methylation profiles
- CpG Islands
- Differentially Methylation Regions (DMRs)
Secondary Analysis
•DMRs associated genes
•Gene set enrichment analysis
Comparative Analysis
Sample Type
Enriched DNA Sample
Required Amount ≥ 50 ng (Concentration ≥ 2ng/μL)
Fragment size - 100 bp~500bp
Purity - OD 260/280 = 1.8 - 2.0