Introduction

Epigenetic regulation includes modulating the transcriptional accessibility of genes through nucleosome positioning and chromatin remodeling.  ATAC-Seq is a rapid assay that allows analysis of epigenetic profiles across the genome by identification of regions that have open or accessible chromatin states. A good alternative to ChIP-seq when beginning epigenetic studies is Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq). ATAC-seq is a common assay being utilized to survey the landscape genome-wide of chromatin assembly, understand accessibility to regions, discover transcription factor binding sites, gene regulation, and more. ATAC-seq has an advantage over other epigenomic assays as it requires a small amount of starting input. Molsys Scientific now offers the entire ATAC-Seq workflow, from library preparation to data analysis.  ATAC-Seq has a higher signal-to-noise ratio and lower input requirements than other similar approaches.

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Applications

•Global view of gene activity

•Novel gene and splice variant discovery

•Chromosomal rearrangement analysis

•Whole mRNA transcript expression analysis

 

Data Analysis

Primary Analysis

•Global methylation profiles

•Specific methylation profiles

  - CpG Islands

  - Differentially Methylation Regions (DMRs)

Secondary Analysis

•DMRs associated genes

•Gene set enrichment analysis

Comparative Analysis

Sample Type

Enriched DNA Sample

Required Amount ≥ 50 ng (Concentration ≥ 2ng/μL)

Fragment size - 100 bp~500bp

Purity  - OD 260/280 = 1.8 - 2.0